polyclonal antibody anti ten 2 Search Results


biotinylated secondary antibody  (Vector Laboratories)
96
Vector Laboratories biotinylated secondary antibody
Biotinylated Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated secondary antibody/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
biotinylated secondary antibody - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier
antibody anti ten 2  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology antibody anti ten 2
Antibody Anti Ten 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody anti ten 2/product/Santa Cruz Biotechnology
Average 94 stars, based on 1 article reviews
antibody anti ten 2 - by Bioz Stars, 2026-04
94/100 stars
  Buy from Supplier
primary polyclonal antibody anti-ten-1-4  (Abnova)
90
Abnova primary polyclonal antibody anti-ten-1-4
Primary Polyclonal Antibody Anti Ten 1 4, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary polyclonal antibody anti-ten-1-4/product/Abnova
Average 90 stars, based on 1 article reviews
primary polyclonal antibody anti-ten-1-4 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
polyclonal antibody anti ten 2  (R&D Systems)
93
R&D Systems polyclonal antibody anti ten 2
Polyclonal Antibody Anti Ten 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal antibody anti ten 2/product/R&D Systems
Average 93 stars, based on 1 article reviews
polyclonal antibody anti ten 2 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
sheep anti ten2 n terminus  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology sheep anti ten2 n terminus
( A ) Recombinant Lasso constructs used in this work (FS, full size). The three proteolytic cleavage sites and the SS splice site are indicated. The antibody recognition sites/epitopes are shown by bars above the structure. Scale bar, 200 amino acids. ( B ) Intracellular processing and release of TENs. Left, <t>TEN2</t> is constitutively cleaved in the trans-Golgi vesicles by furin at site 1. Middle, when delivered to the cell surface, the ECD remains tethered to the membrane and functions as a cell-surface receptor. Right, regulated cleavage at site 3 releases the ECD into the medium. ( C ) Expression of Lasso and release of its ECD fragment in hippocampal neurons in culture. Rat hippocampal neurons were cultured for 3, 7 and 14 days, and proportionate amounts of the conditioned media and cell lysates were separated by SDS-PAGE. A Western blot (representative of three independent experiments, which all gave similar results) was stained for Lasso, LPHN1, neurofilament-H (NF-H), and actinin. The doublet bands corresponding to splice variants of full-size Lasso (FS) and the fragment of ECD (Frag.) cleaved at site 1 are indicated by arrowheads. ( D ) Quantification of Western blots (as in C), using Lasso C-terminus staining data. ( E ) Axonal growth cones (white arrowheads) do not express Lasso/teneurin-2. Neurons in a 9 DIV hippocampal culture were permeabilized and stained for the axonal protein Tau (green) and Lasso (TN2C, red) (representative image from n = 5 experiments). ( F ) A detailed study of growth cones. Hippocampal neurons were transfected with a vector encoding GFP, then, after 14 DIV, stained for LPHN1 (PAL1 and Alexa 647-conjugated secondary antibody, magenta), and axonal growth cones were visualized by GFP fluorescence (green). ( G, H ) Correlation of LPHN1 polarization within a growth cone with its recent travel trajectory. G left, a fluorescent image of a growth cone stained for LPHN1 (magenta). G right, the same image in false color (contour based on GFP staining), demonstrating LPHN1 polarization on the right side. H left, the contours of 13 roughly symmetrical growth cones and their preceding axons were aligned to locate the stronger LPHN1 staining on the right. Note, that all axons approach growth cones from the right low quadrant. H right, the proportion of right- and left-turning growth cones plotted with Jeffreys 99.73% confidence intervals for a binomial parameter; ***, p<0.001; n = 13. ( I ). LPHN1 is found within filopodia and lamellipodia on the leading edge (left, arrowheads), but not on the trailing edge (right) of a growth cone. Green, GFP fluorescence; magenta, PAL1 staining for LPHN1. 10.7554/eLife.37935.004 Figure 1—source data 1. Source data for , Panels D and H.
Sheep Anti Ten2 N Terminus, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sheep anti ten2 n terminus/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
sheep anti ten2 n terminus - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
biotinylated secondary antibody  (Santa Cruz Biotechnology)
98
Santa Cruz Biotechnology biotinylated secondary antibody
( A ) Recombinant Lasso constructs used in this work (FS, full size). The three proteolytic cleavage sites and the SS splice site are indicated. The antibody recognition sites/epitopes are shown by bars above the structure. Scale bar, 200 amino acids. ( B ) Intracellular processing and release of TENs. Left, <t>TEN2</t> is constitutively cleaved in the trans-Golgi vesicles by furin at site 1. Middle, when delivered to the cell surface, the ECD remains tethered to the membrane and functions as a cell-surface receptor. Right, regulated cleavage at site 3 releases the ECD into the medium. ( C ) Expression of Lasso and release of its ECD fragment in hippocampal neurons in culture. Rat hippocampal neurons were cultured for 3, 7 and 14 days, and proportionate amounts of the conditioned media and cell lysates were separated by SDS-PAGE. A Western blot (representative of three independent experiments, which all gave similar results) was stained for Lasso, LPHN1, neurofilament-H (NF-H), and actinin. The doublet bands corresponding to splice variants of full-size Lasso (FS) and the fragment of ECD (Frag.) cleaved at site 1 are indicated by arrowheads. ( D ) Quantification of Western blots (as in C), using Lasso C-terminus staining data. ( E ) Axonal growth cones (white arrowheads) do not express Lasso/teneurin-2. Neurons in a 9 DIV hippocampal culture were permeabilized and stained for the axonal protein Tau (green) and Lasso (TN2C, red) (representative image from n = 5 experiments). ( F ) A detailed study of growth cones. Hippocampal neurons were transfected with a vector encoding GFP, then, after 14 DIV, stained for LPHN1 (PAL1 and Alexa 647-conjugated secondary antibody, magenta), and axonal growth cones were visualized by GFP fluorescence (green). ( G, H ) Correlation of LPHN1 polarization within a growth cone with its recent travel trajectory. G left, a fluorescent image of a growth cone stained for LPHN1 (magenta). G right, the same image in false color (contour based on GFP staining), demonstrating LPHN1 polarization on the right side. H left, the contours of 13 roughly symmetrical growth cones and their preceding axons were aligned to locate the stronger LPHN1 staining on the right. Note, that all axons approach growth cones from the right low quadrant. H right, the proportion of right- and left-turning growth cones plotted with Jeffreys 99.73% confidence intervals for a binomial parameter; ***, p<0.001; n = 13. ( I ). LPHN1 is found within filopodia and lamellipodia on the leading edge (left, arrowheads), but not on the trailing edge (right) of a growth cone. Green, GFP fluorescence; magenta, PAL1 staining for LPHN1. 10.7554/eLife.37935.004 Figure 1—source data 1. Source data for , Panels D and H.
Biotinylated Secondary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated secondary antibody/product/Santa Cruz Biotechnology
Average 98 stars, based on 1 article reviews
biotinylated secondary antibody - by Bioz Stars, 2026-04
98/100 stars
  Buy from Supplier
polyclonal antibody anti ten 2  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology polyclonal antibody anti ten 2
( A ) Recombinant Lasso constructs used in this work (FS, full size). The three proteolytic cleavage sites and the SS splice site are indicated. The antibody recognition sites/epitopes are shown by bars above the structure. Scale bar, 200 amino acids. ( B ) Intracellular processing and release of TENs. Left, <t>TEN2</t> is constitutively cleaved in the trans-Golgi vesicles by furin at site 1. Middle, when delivered to the cell surface, the ECD remains tethered to the membrane and functions as a cell-surface receptor. Right, regulated cleavage at site 3 releases the ECD into the medium. ( C ) Expression of Lasso and release of its ECD fragment in hippocampal neurons in culture. Rat hippocampal neurons were cultured for 3, 7 and 14 days, and proportionate amounts of the conditioned media and cell lysates were separated by SDS-PAGE. A Western blot (representative of three independent experiments, which all gave similar results) was stained for Lasso, LPHN1, neurofilament-H (NF-H), and actinin. The doublet bands corresponding to splice variants of full-size Lasso (FS) and the fragment of ECD (Frag.) cleaved at site 1 are indicated by arrowheads. ( D ) Quantification of Western blots (as in C), using Lasso C-terminus staining data. ( E ) Axonal growth cones (white arrowheads) do not express Lasso/teneurin-2. Neurons in a 9 DIV hippocampal culture were permeabilized and stained for the axonal protein Tau (green) and Lasso (TN2C, red) (representative image from n = 5 experiments). ( F ) A detailed study of growth cones. Hippocampal neurons were transfected with a vector encoding GFP, then, after 14 DIV, stained for LPHN1 (PAL1 and Alexa 647-conjugated secondary antibody, magenta), and axonal growth cones were visualized by GFP fluorescence (green). ( G, H ) Correlation of LPHN1 polarization within a growth cone with its recent travel trajectory. G left, a fluorescent image of a growth cone stained for LPHN1 (magenta). G right, the same image in false color (contour based on GFP staining), demonstrating LPHN1 polarization on the right side. H left, the contours of 13 roughly symmetrical growth cones and their preceding axons were aligned to locate the stronger LPHN1 staining on the right. Note, that all axons approach growth cones from the right low quadrant. H right, the proportion of right- and left-turning growth cones plotted with Jeffreys 99.73% confidence intervals for a binomial parameter; ***, p<0.001; n = 13. ( I ). LPHN1 is found within filopodia and lamellipodia on the leading edge (left, arrowheads), but not on the trailing edge (right) of a growth cone. Green, GFP fluorescence; magenta, PAL1 staining for LPHN1. 10.7554/eLife.37935.004 Figure 1—source data 1. Source data for , Panels D and H.
Polyclonal Antibody Anti Ten 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal antibody anti ten 2/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
polyclonal antibody anti ten 2 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
imagequant las 500  (GE Healthcare)
96
GE Healthcare imagequant las 500
( A ) Recombinant Lasso constructs used in this work (FS, full size). The three proteolytic cleavage sites and the SS splice site are indicated. The antibody recognition sites/epitopes are shown by bars above the structure. Scale bar, 200 amino acids. ( B ) Intracellular processing and release of TENs. Left, <t>TEN2</t> is constitutively cleaved in the trans-Golgi vesicles by furin at site 1. Middle, when delivered to the cell surface, the ECD remains tethered to the membrane and functions as a cell-surface receptor. Right, regulated cleavage at site 3 releases the ECD into the medium. ( C ) Expression of Lasso and release of its ECD fragment in hippocampal neurons in culture. Rat hippocampal neurons were cultured for 3, 7 and 14 days, and proportionate amounts of the conditioned media and cell lysates were separated by SDS-PAGE. A Western blot (representative of three independent experiments, which all gave similar results) was stained for Lasso, LPHN1, neurofilament-H (NF-H), and actinin. The doublet bands corresponding to splice variants of full-size Lasso (FS) and the fragment of ECD (Frag.) cleaved at site 1 are indicated by arrowheads. ( D ) Quantification of Western blots (as in C), using Lasso C-terminus staining data. ( E ) Axonal growth cones (white arrowheads) do not express Lasso/teneurin-2. Neurons in a 9 DIV hippocampal culture were permeabilized and stained for the axonal protein Tau (green) and Lasso (TN2C, red) (representative image from n = 5 experiments). ( F ) A detailed study of growth cones. Hippocampal neurons were transfected with a vector encoding GFP, then, after 14 DIV, stained for LPHN1 (PAL1 and Alexa 647-conjugated secondary antibody, magenta), and axonal growth cones were visualized by GFP fluorescence (green). ( G, H ) Correlation of LPHN1 polarization within a growth cone with its recent travel trajectory. G left, a fluorescent image of a growth cone stained for LPHN1 (magenta). G right, the same image in false color (contour based on GFP staining), demonstrating LPHN1 polarization on the right side. H left, the contours of 13 roughly symmetrical growth cones and their preceding axons were aligned to locate the stronger LPHN1 staining on the right. Note, that all axons approach growth cones from the right low quadrant. H right, the proportion of right- and left-turning growth cones plotted with Jeffreys 99.73% confidence intervals for a binomial parameter; ***, p<0.001; n = 13. ( I ). LPHN1 is found within filopodia and lamellipodia on the leading edge (left, arrowheads), but not on the trailing edge (right) of a growth cone. Green, GFP fluorescence; magenta, PAL1 staining for LPHN1. 10.7554/eLife.37935.004 Figure 1—source data 1. Source data for , Panels D and H.
Imagequant Las 500, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/imagequant las 500/product/GE Healthcare
Average 96 stars, based on 1 article reviews
imagequant las 500 - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier
mouse anti his antibody  (Qiagen)
97
Qiagen mouse anti his antibody
( A ) Recombinant Lasso constructs used in this work (FS, full size). The three proteolytic cleavage sites and the SS splice site are indicated. The antibody recognition sites/epitopes are shown by bars above the structure. Scale bar, 200 amino acids. ( B ) Intracellular processing and release of TENs. Left, <t>TEN2</t> is constitutively cleaved in the trans-Golgi vesicles by furin at site 1. Middle, when delivered to the cell surface, the ECD remains tethered to the membrane and functions as a cell-surface receptor. Right, regulated cleavage at site 3 releases the ECD into the medium. ( C ) Expression of Lasso and release of its ECD fragment in hippocampal neurons in culture. Rat hippocampal neurons were cultured for 3, 7 and 14 days, and proportionate amounts of the conditioned media and cell lysates were separated by SDS-PAGE. A Western blot (representative of three independent experiments, which all gave similar results) was stained for Lasso, LPHN1, neurofilament-H (NF-H), and actinin. The doublet bands corresponding to splice variants of full-size Lasso (FS) and the fragment of ECD (Frag.) cleaved at site 1 are indicated by arrowheads. ( D ) Quantification of Western blots (as in C), using Lasso C-terminus staining data. ( E ) Axonal growth cones (white arrowheads) do not express Lasso/teneurin-2. Neurons in a 9 DIV hippocampal culture were permeabilized and stained for the axonal protein Tau (green) and Lasso (TN2C, red) (representative image from n = 5 experiments). ( F ) A detailed study of growth cones. Hippocampal neurons were transfected with a vector encoding GFP, then, after 14 DIV, stained for LPHN1 (PAL1 and Alexa 647-conjugated secondary antibody, magenta), and axonal growth cones were visualized by GFP fluorescence (green). ( G, H ) Correlation of LPHN1 polarization within a growth cone with its recent travel trajectory. G left, a fluorescent image of a growth cone stained for LPHN1 (magenta). G right, the same image in false color (contour based on GFP staining), demonstrating LPHN1 polarization on the right side. H left, the contours of 13 roughly symmetrical growth cones and their preceding axons were aligned to locate the stronger LPHN1 staining on the right. Note, that all axons approach growth cones from the right low quadrant. H right, the proportion of right- and left-turning growth cones plotted with Jeffreys 99.73% confidence intervals for a binomial parameter; ***, p<0.001; n = 13. ( I ). LPHN1 is found within filopodia and lamellipodia on the leading edge (left, arrowheads), but not on the trailing edge (right) of a growth cone. Green, GFP fluorescence; magenta, PAL1 staining for LPHN1. 10.7554/eLife.37935.004 Figure 1—source data 1. Source data for , Panels D and H.
Mouse Anti His Antibody, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti his antibody/product/Qiagen
Average 97 stars, based on 1 article reviews
mouse anti his antibody - by Bioz Stars, 2026-04
97/100 stars
  Buy from Supplier
rabbit anti ha  (Thermo Fisher)
86
Thermo Fisher rabbit anti ha
( A ) Recombinant Lasso constructs used in this work (FS, full size). The three proteolytic cleavage sites and the SS splice site are indicated. The antibody recognition sites/epitopes are shown by bars above the structure. Scale bar, 200 amino acids. ( B ) Intracellular processing and release of TENs. Left, <t>TEN2</t> is constitutively cleaved in the trans-Golgi vesicles by furin at site 1. Middle, when delivered to the cell surface, the ECD remains tethered to the membrane and functions as a cell-surface receptor. Right, regulated cleavage at site 3 releases the ECD into the medium. ( C ) Expression of Lasso and release of its ECD fragment in hippocampal neurons in culture. Rat hippocampal neurons were cultured for 3, 7 and 14 days, and proportionate amounts of the conditioned media and cell lysates were separated by SDS-PAGE. A Western blot (representative of three independent experiments, which all gave similar results) was stained for Lasso, LPHN1, neurofilament-H (NF-H), and actinin. The doublet bands corresponding to splice variants of full-size Lasso (FS) and the fragment of ECD (Frag.) cleaved at site 1 are indicated by arrowheads. ( D ) Quantification of Western blots (as in C), using Lasso C-terminus staining data. ( E ) Axonal growth cones (white arrowheads) do not express Lasso/teneurin-2. Neurons in a 9 DIV hippocampal culture were permeabilized and stained for the axonal protein Tau (green) and Lasso (TN2C, red) (representative image from n = 5 experiments). ( F ) A detailed study of growth cones. Hippocampal neurons were transfected with a vector encoding GFP, then, after 14 DIV, stained for LPHN1 (PAL1 and Alexa 647-conjugated secondary antibody, magenta), and axonal growth cones were visualized by GFP fluorescence (green). ( G, H ) Correlation of LPHN1 polarization within a growth cone with its recent travel trajectory. G left, a fluorescent image of a growth cone stained for LPHN1 (magenta). G right, the same image in false color (contour based on GFP staining), demonstrating LPHN1 polarization on the right side. H left, the contours of 13 roughly symmetrical growth cones and their preceding axons were aligned to locate the stronger LPHN1 staining on the right. Note, that all axons approach growth cones from the right low quadrant. H right, the proportion of right- and left-turning growth cones plotted with Jeffreys 99.73% confidence intervals for a binomial parameter; ***, p<0.001; n = 13. ( I ). LPHN1 is found within filopodia and lamellipodia on the leading edge (left, arrowheads), but not on the trailing edge (right) of a growth cone. Green, GFP fluorescence; magenta, PAL1 staining for LPHN1. 10.7554/eLife.37935.004 Figure 1—source data 1. Source data for , Panels D and H.
Rabbit Anti Ha, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti ha/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
rabbit anti ha - by Bioz Stars, 2026-04
86/100 stars
  Buy from Supplier
mouse anti-flag m2 f3165  (Millipore)
90
Millipore mouse anti-flag m2 f3165
( A ) Recombinant Lasso constructs used in this work (FS, full size). The three proteolytic cleavage sites and the SS splice site are indicated. The antibody recognition sites/epitopes are shown by bars above the structure. Scale bar, 200 amino acids. ( B ) Intracellular processing and release of TENs. Left, <t>TEN2</t> is constitutively cleaved in the trans-Golgi vesicles by furin at site 1. Middle, when delivered to the cell surface, the ECD remains tethered to the membrane and functions as a cell-surface receptor. Right, regulated cleavage at site 3 releases the ECD into the medium. ( C ) Expression of Lasso and release of its ECD fragment in hippocampal neurons in culture. Rat hippocampal neurons were cultured for 3, 7 and 14 days, and proportionate amounts of the conditioned media and cell lysates were separated by SDS-PAGE. A Western blot (representative of three independent experiments, which all gave similar results) was stained for Lasso, LPHN1, neurofilament-H (NF-H), and actinin. The doublet bands corresponding to splice variants of full-size Lasso (FS) and the fragment of ECD (Frag.) cleaved at site 1 are indicated by arrowheads. ( D ) Quantification of Western blots (as in C), using Lasso C-terminus staining data. ( E ) Axonal growth cones (white arrowheads) do not express Lasso/teneurin-2. Neurons in a 9 DIV hippocampal culture were permeabilized and stained for the axonal protein Tau (green) and Lasso (TN2C, red) (representative image from n = 5 experiments). ( F ) A detailed study of growth cones. Hippocampal neurons were transfected with a vector encoding GFP, then, after 14 DIV, stained for LPHN1 (PAL1 and Alexa 647-conjugated secondary antibody, magenta), and axonal growth cones were visualized by GFP fluorescence (green). ( G, H ) Correlation of LPHN1 polarization within a growth cone with its recent travel trajectory. G left, a fluorescent image of a growth cone stained for LPHN1 (magenta). G right, the same image in false color (contour based on GFP staining), demonstrating LPHN1 polarization on the right side. H left, the contours of 13 roughly symmetrical growth cones and their preceding axons were aligned to locate the stronger LPHN1 staining on the right. Note, that all axons approach growth cones from the right low quadrant. H right, the proportion of right- and left-turning growth cones plotted with Jeffreys 99.73% confidence intervals for a binomial parameter; ***, p<0.001; n = 13. ( I ). LPHN1 is found within filopodia and lamellipodia on the leading edge (left, arrowheads), but not on the trailing edge (right) of a growth cone. Green, GFP fluorescence; magenta, PAL1 staining for LPHN1. 10.7554/eLife.37935.004 Figure 1—source data 1. Source data for , Panels D and H.
Mouse Anti Flag M2 F3165, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-flag m2 f3165/product/Millipore
Average 90 stars, based on 1 article reviews
mouse anti-flag m2 f3165 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
mouse monoclonal anti-myc clone 9e10 05–419  (Millipore)
90
Millipore mouse monoclonal anti-myc clone 9e10 05–419

Mouse Monoclonal Anti Myc Clone 9e10 05–419, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti-myc clone 9e10 05–419/product/Millipore
Average 90 stars, based on 1 article reviews
mouse monoclonal anti-myc clone 9e10 05–419 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


( A ) Recombinant Lasso constructs used in this work (FS, full size). The three proteolytic cleavage sites and the SS splice site are indicated. The antibody recognition sites/epitopes are shown by bars above the structure. Scale bar, 200 amino acids. ( B ) Intracellular processing and release of TENs. Left, TEN2 is constitutively cleaved in the trans-Golgi vesicles by furin at site 1. Middle, when delivered to the cell surface, the ECD remains tethered to the membrane and functions as a cell-surface receptor. Right, regulated cleavage at site 3 releases the ECD into the medium. ( C ) Expression of Lasso and release of its ECD fragment in hippocampal neurons in culture. Rat hippocampal neurons were cultured for 3, 7 and 14 days, and proportionate amounts of the conditioned media and cell lysates were separated by SDS-PAGE. A Western blot (representative of three independent experiments, which all gave similar results) was stained for Lasso, LPHN1, neurofilament-H (NF-H), and actinin. The doublet bands corresponding to splice variants of full-size Lasso (FS) and the fragment of ECD (Frag.) cleaved at site 1 are indicated by arrowheads. ( D ) Quantification of Western blots (as in C), using Lasso C-terminus staining data. ( E ) Axonal growth cones (white arrowheads) do not express Lasso/teneurin-2. Neurons in a 9 DIV hippocampal culture were permeabilized and stained for the axonal protein Tau (green) and Lasso (TN2C, red) (representative image from n = 5 experiments). ( F ) A detailed study of growth cones. Hippocampal neurons were transfected with a vector encoding GFP, then, after 14 DIV, stained for LPHN1 (PAL1 and Alexa 647-conjugated secondary antibody, magenta), and axonal growth cones were visualized by GFP fluorescence (green). ( G, H ) Correlation of LPHN1 polarization within a growth cone with its recent travel trajectory. G left, a fluorescent image of a growth cone stained for LPHN1 (magenta). G right, the same image in false color (contour based on GFP staining), demonstrating LPHN1 polarization on the right side. H left, the contours of 13 roughly symmetrical growth cones and their preceding axons were aligned to locate the stronger LPHN1 staining on the right. Note, that all axons approach growth cones from the right low quadrant. H right, the proportion of right- and left-turning growth cones plotted with Jeffreys 99.73% confidence intervals for a binomial parameter; ***, p<0.001; n = 13. ( I ). LPHN1 is found within filopodia and lamellipodia on the leading edge (left, arrowheads), but not on the trailing edge (right) of a growth cone. Green, GFP fluorescence; magenta, PAL1 staining for LPHN1. 10.7554/eLife.37935.004 Figure 1—source data 1. Source data for , Panels D and H.

Journal: eLife

Article Title: Proteolytically released Lasso/teneurin-2 induces axonal attraction by interacting with latrophilin-1 on axonal growth cones

doi: 10.7554/eLife.37935

Figure Lengend Snippet: ( A ) Recombinant Lasso constructs used in this work (FS, full size). The three proteolytic cleavage sites and the SS splice site are indicated. The antibody recognition sites/epitopes are shown by bars above the structure. Scale bar, 200 amino acids. ( B ) Intracellular processing and release of TENs. Left, TEN2 is constitutively cleaved in the trans-Golgi vesicles by furin at site 1. Middle, when delivered to the cell surface, the ECD remains tethered to the membrane and functions as a cell-surface receptor. Right, regulated cleavage at site 3 releases the ECD into the medium. ( C ) Expression of Lasso and release of its ECD fragment in hippocampal neurons in culture. Rat hippocampal neurons were cultured for 3, 7 and 14 days, and proportionate amounts of the conditioned media and cell lysates were separated by SDS-PAGE. A Western blot (representative of three independent experiments, which all gave similar results) was stained for Lasso, LPHN1, neurofilament-H (NF-H), and actinin. The doublet bands corresponding to splice variants of full-size Lasso (FS) and the fragment of ECD (Frag.) cleaved at site 1 are indicated by arrowheads. ( D ) Quantification of Western blots (as in C), using Lasso C-terminus staining data. ( E ) Axonal growth cones (white arrowheads) do not express Lasso/teneurin-2. Neurons in a 9 DIV hippocampal culture were permeabilized and stained for the axonal protein Tau (green) and Lasso (TN2C, red) (representative image from n = 5 experiments). ( F ) A detailed study of growth cones. Hippocampal neurons were transfected with a vector encoding GFP, then, after 14 DIV, stained for LPHN1 (PAL1 and Alexa 647-conjugated secondary antibody, magenta), and axonal growth cones were visualized by GFP fluorescence (green). ( G, H ) Correlation of LPHN1 polarization within a growth cone with its recent travel trajectory. G left, a fluorescent image of a growth cone stained for LPHN1 (magenta). G right, the same image in false color (contour based on GFP staining), demonstrating LPHN1 polarization on the right side. H left, the contours of 13 roughly symmetrical growth cones and their preceding axons were aligned to locate the stronger LPHN1 staining on the right. Note, that all axons approach growth cones from the right low quadrant. H right, the proportion of right- and left-turning growth cones plotted with Jeffreys 99.73% confidence intervals for a binomial parameter; ***, p<0.001; n = 13. ( I ). LPHN1 is found within filopodia and lamellipodia on the leading edge (left, arrowheads), but not on the trailing edge (right) of a growth cone. Green, GFP fluorescence; magenta, PAL1 staining for LPHN1. 10.7554/eLife.37935.004 Figure 1—source data 1. Source data for , Panels D and H.

Article Snippet: The following antibodies were used in this work: Rabbit anti-NF-H (Neuromics, RA22116); mouse anti-MAP-2 (Neuromics, MO22116); mouse monoclonal anti-V5 (clone SV5-Pk1, AbD Serotec/Bio-Rad, MCA1360); rabbit anti-V5 (Thermo Fisher Scientific, PA1-29324; RRID: AB_1961277 ); mouse monoclonal anti-myc (clone 9E10, Millipore, 05–419; RRID: AB_309725 ); chicken anti-myc (Millipore, AB3252; RRID: AB_2235702 ); mouse anti-FLAG M2 (Sigma-Aldrich, F3165; RRID: AB_259529 ); anti-FLAG M2 affinity gel (Sigma-Aldrich, A2220); mouse anti-actinin (Sigma-Aldrich, A7811); rabbit polyclonal anti-LPHN1-peptide (PAL1, ( ); rabbit polyclonal anti-LPHN1 NTF (RL1) ( ); mouse anti-Lasso/TEN2 C-terminus (TN2C, dmAb) ( ); sheep anti-TEN2 N-terminus (TN2N, R and D systems, AF4578; RRID: AB_10719438 ); mouse anti-synapsin (Santa-Cruz Biotechnology, sc-376623; RRID: AB_11150313 ); rabbit anti-PSD-95 (Millipore, AB9708; RRID: AB_11212529 ); rabbit anti-Tau (Synaptic Systems, 314 002; RRID: AB_993042 ); rabbit anti-GFP (Thermo Fisher Scientific, A-11122; RRID: AB_221569 ).

Techniques: Recombinant, Construct, Cell Surface Receptor Assay, Expressing, Cell Culture, SDS Page, Western Blot, Staining, Transfection, Plasmid Preparation, Fluorescence

Journal: eLife

Article Title: Proteolytically released Lasso/teneurin-2 induces axonal attraction by interacting with latrophilin-1 on axonal growth cones

doi: 10.7554/eLife.37935

Figure Lengend Snippet:

Article Snippet: The following antibodies were used in this work: Rabbit anti-NF-H (Neuromics, RA22116); mouse anti-MAP-2 (Neuromics, MO22116); mouse monoclonal anti-V5 (clone SV5-Pk1, AbD Serotec/Bio-Rad, MCA1360); rabbit anti-V5 (Thermo Fisher Scientific, PA1-29324; RRID: AB_1961277 ); mouse monoclonal anti-myc (clone 9E10, Millipore, 05–419; RRID: AB_309725 ); chicken anti-myc (Millipore, AB3252; RRID: AB_2235702 ); mouse anti-FLAG M2 (Sigma-Aldrich, F3165; RRID: AB_259529 ); anti-FLAG M2 affinity gel (Sigma-Aldrich, A2220); mouse anti-actinin (Sigma-Aldrich, A7811); rabbit polyclonal anti-LPHN1-peptide (PAL1, ( ); rabbit polyclonal anti-LPHN1 NTF (RL1) ( ); mouse anti-Lasso/TEN2 C-terminus (TN2C, dmAb) ( ); sheep anti-TEN2 N-terminus (TN2N, R and D systems, AF4578; RRID: AB_10719438 ); mouse anti-synapsin (Santa-Cruz Biotechnology, sc-376623; RRID: AB_11150313 ); rabbit anti-PSD-95 (Millipore, AB9708; RRID: AB_11212529 ); rabbit anti-Tau (Synaptic Systems, 314 002; RRID: AB_993042 ); rabbit anti-GFP (Thermo Fisher Scientific, A-11122; RRID: AB_221569 ).

Techniques: Immunocytochemistry, Western Blot, Purification, Isolation, Recombinant, Blocking Assay, Expressing, Sequencing, Negative Control, Software

Journal: eLife

Article Title: Proteolytically released Lasso/teneurin-2 induces axonal attraction by interacting with latrophilin-1 on axonal growth cones

doi: 10.7554/eLife.37935

Figure Lengend Snippet:

Article Snippet: The following antibodies were used in this work: Rabbit anti-NF-H (Neuromics, RA22116); mouse anti-MAP-2 (Neuromics, MO22116); mouse monoclonal anti-V5 (clone SV5-Pk1, AbD Serotec/Bio-Rad, MCA1360); rabbit anti-V5 (Thermo Fisher Scientific, PA1-29324; RRID: AB_1961277 ); mouse monoclonal anti-myc (clone 9E10, Millipore, 05–419; RRID: AB_309725 ); chicken anti-myc (Millipore, AB3252; RRID: AB_2235702 ); mouse anti-FLAG M2 (Sigma-Aldrich, F3165; RRID: AB_259529 ); anti-FLAG M2 affinity gel (Sigma-Aldrich, A2220); mouse anti-actinin (Sigma-Aldrich, A7811); rabbit polyclonal anti-LPHN1-peptide (PAL1, ( ); rabbit polyclonal anti-LPHN1 NTF (RL1) ( ); mouse anti-Lasso/TEN2 C-terminus (TN2C, dmAb) ( ); sheep anti-TEN2 N-terminus (TN2N, R and D systems, AF4578; RRID: AB_10719438 ); mouse anti-synapsin (Santa-Cruz Biotechnology, sc-376623; RRID: AB_11150313 ); rabbit anti-PSD-95 (Millipore, AB9708; RRID: AB_11212529 ); rabbit anti-Tau (Synaptic Systems, 314 002; RRID: AB_993042 ); rabbit anti-GFP (Thermo Fisher Scientific, A-11122; RRID: AB_221569 ).

Techniques: Immunocytochemistry, Western Blot, Purification, Isolation, Recombinant, Blocking Assay, Expressing, Sequencing, Negative Control, Software